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Cascade cultivation of microorganisms

Investigation of bacterial culture at different growth stages, measurements of kinetics of bacterial growth and product formation, investigation of kinetics of biodegradation and biotransformation processes are typical problems in many scientific studies and in developments of optimized industrial technologies. Traditional fermentation equipment used in such studies is timeconsuming and unprofitable.

The system "Cascade" is designed to reduce experiment time and to improve reproducibility of experiments. The system realizes a new cultivation mode, named cascade cultivation. The fermentation module of the system is composed of a cascade of mini-bioreactors (0.5 to 3 L) sequentialy connected with each other. The system has also sterilization and control modules.

The major principal distinction of the method realized in the "Cascad" system from the traditional methods is that the studied phases of a biological process, for example, points on the microorganism growth curve, constantly exist through all the experiment time. This mode is achieved by means of a cascade flow of the culture liquid through the bioreactors. The time growth curve turns to be unfolded in space and required phases of the process are always accessible for study. A researcher can easily inject various agents, take samples and explore effects of various agents on the process.

Figure 1 shows a biotechnological system with a fermentation module consisting of three 0.5 L bioreactors. Figure 2 demonstrates a fermentation module consisting of three 3L bioreactors mounted in horizontal position.

Figure 1. Biotechnological system "Oka-01-MF-05K" composed of a steam generator PG-20, solution sterilizer SR-10, gas sterilizer SG-3, autoclave AK-25, fermentation module MF-05K (cascade) and a control module with a controller MU-3K.

Figure 2. Fermentation module MF-3K of the biotechnological system of the "Oka-02" series.

Specification for the biotechnological system "Oka-01-MF-3K"

Power sources
Electric mains voltage 380/220 V
frequency 50 Hz
Compressed air pressure 0,2 MPa
flow rate 5 L/min
Main water pressure 0,2 MPa
flow rate 5 L/min
Electric power 18 kW
Sterilization module (cyclic sterilization)
Steam generator volume 25 L
" Maximal steam temperature 130ºC
Maximal steam pressure 0,15 MPa
Maximal steam flow 5 kg/h
Volume of the sterilization chamber 10 L
Volume of the dispensing apparatus 1 L
Maximal flow of steril medium 10 L/h
Three-channel gas sterilizer incoming, exhaust, and technological gases
Autoclave volume 25 L
Mode of operation " Continuous
Control Processor controled
Fermentation module of the cascade type MF-3K
Bioreactor volume 3 L
Load fact 0,5-0,6
Agitation pneumohydravlic
Sterilization by steam
Temperature control from external thermostat
Modes of operation periodic, semicontinuous
Operating conditions aseptic
Interactive control module
Microprocessor type Signaling
Aeration type pneumohydravlic aeration
Control of aeration Frequency control
Temperature measurement 10 to 150ºC
Measurement error ±0,2ºC
Channels of measurements amount 8
Temperature sensor resistance thermometer
pH measurement от 0 до 15
Measurement error ±0,05
Channels of measurements amount 3
Sensor type Mettler Toledo
pO2 measurements 0 to 100
Channels of measurements amount 3
Sensor type Mettler Toledo
Number of outputs 10
Output signal voltage 24 V
Permissible current 100 mA
Electric mains Power supply 380/220 V
Frequency 50 Hz
Power 100 W

Biotechnological system "Oka-02-MF-3K"

  • Steam generator PG-20;
  • Gas sterilizer SG-3;
  • Solution sterilizer SR-10;
  • Autoclave AK-25;
  • Fermentation module MF-3K.
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